Employee self-efficacy is a critical psychological factor that influences employees' confidence, motivation, adaptability, and job performance in organizational settings. The present study examines the influence of work culture on employee self-efficacy among employees of State Bank of India (SBI) branches in Central Kerala. A quantitative research approach employing descriptive and explanatory research designs was adopted for the study. Primary data were collected from 248 SBI employees using a structured questionnaire comprising measures of work culture and employee self-efficacy. Descriptive statistics were used to assess the levels of work culture and self-efficacy, while simple linear regression analysis was employed to determine the influence of work culture on employee self-efficacy. The findings revealed that employees perceived the work culture in SBI branches to be moderately high and exhibited a moderate to high level of self-efficacy. The regression results indicated that work culture has a significant positive influence on employee self-efficacy (β = 0.612, p < 0.001), explaining 29.4% of the variance in self-efficacy (R² = 0.294). The study concludes that a supportive work culture characterized by effective communication, teamwork, leadership support, and employee involvement enhances employees' confidence in their ability to perform job-related tasks effectively. The findings provide valuable insights for banking administrators and human resource managers in fostering a positive work environment that strengthens employee self-efficacy and organizational effectiveness.