Ecotourism represents a sustainable development approach that fosters environmental conservation while generating economic and social benefits for local communities. In the context of Manipur—a state in Northeast India known for its rich biodiversity, scenic landscapes, and indigenous cultures, ecotourism holds significant potential. This research aims to identify key stakeholders in Manipur's ecotourism development, explore their roles and contributions, and evaluate the opportunities and challenges they face. The study is descriptive in nature utilizing secondary data collected from government reports, policy documents, tourism department statistics, academic journals, media sources, etc. Diverse stakeholders, including local communities, government, NGOs, private sectors, and tourists, actively shape Manipur's ecotourism with varying involvement levels, led by communities and authorities. Initiatives like homestays, policies, campaigns, and infrastructure promote empowerment and sustainability but are hindered by bureaucracy, limited access, security, and environmental strains such as waste and encroachment. Collaborative governance with enhanced coordination and community management is vital for meeting tourist expectations and securing long-term benefits. Stakeholder mapping highlights key ecotourism players in Manipur local communities, NGOs, academics, private sector, government contributing to heritage, training, investment, and policies amid coordination and funding challenges. Opportunities include biodiversity-driven homestays, trails, partnerships, and digital cultural marketing. Recommendations urge multi-stakeholder coordination, capacity-building, equitable policies, and eco-infrastructure for sustainable economic, cultural, and environmental growth.